Sources

Generating human intestinal tissue from pluripotent stem cells in vitro

Generated 2026-04-12 07:16 UTC

Metadata

title
Generating human intestinal tissue from pluripotent stem cells in vitro
kind
paper
status
ingested
added
2026-04-08T15:46:54+09:00
raw source
raw/sources/mccracken_2011_generating_human_intestinal_tissue_from.pdf
article url
https://www.nature.com/articles/nprot.2011.410
published date
2011-11-10
organ
intestine
protocol focus
foundational intestinal organoid generation from hPSCs
deep ingested
2026-04-08

Source

Study design

  • Starting material: human pluripotent stem cells
  • Protocol type: stepwise derivation and maturation protocol
  • Aim: foundational intestinal organoid generation from hPSCs
  • Core readouts: organoid morphology, lineage markers, and downstream functional assays

Summary

  • This paper is best understood as a stepwise derivation and maturation protocol for foundational intestinal organoid generation from hPSCs.
  • Its main distinctive contribution in this corpus is that it builds a stepwise hPSC-to-endoderm-to-mid/hindgut route that turns gut spheroids into intestinal organoids.
  • Within this collection, it belongs to the baseline derivation branch of organoid protocol work.
  • Paper framing: Here we describe a protocol for generating 3D human intestinal tissues (called organoids) in vitro from human pluripotent stem cells (hPSCs). To generate intestinal organoids, pluripotent stem cells are first differentiated into FOXA2 + SOX17 + endoderm by treating the cells with activin A for 3 d.

Key findings

  • Defines a workflow centered on foundational intestinal organoid generation from hPSCs.
  • Its distinctive focus in practice is the way it builds a stepwise hPSC-to-endoderm-to-mid/hindgut route that turns gut spheroids into intestinal organoids.
  • Serves as a baseline generation protocol that other assay, maturation, or perturbation papers can build on.

Strengths

  • Useful as a starting-point protocol for building this organ system from stem cells.
  • Makes lineage commitments and media transitions explicit enough to anchor comparison across later protocols.

Limitations and caveats

  • Still likely to depend on stem-cell line quality, timing precision, and local optimization.
  • Baseline derivation protocols often need additional maturation or assay layers before they answer higher-order biological questions.

Relevance to this corpus

  • Specific role in this corpus: Foundational entry point for endodermal organoid work in this collection.
  • This paper broadens the collection's coverage of intestine organoid work.
  • It is most valuable as a baseline protocol to compare against later assay, maturation, or refinement papers.

Open questions

  • Which steps in this intestine workflow drive the most variability across lines or batches?
  • What extra maturation or assay layer is usually needed after the baseline derivation works?

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