Current position in this corpus
The endodermal side of the corpus spans both foundational derivation and later assay engineering, from gut and gastric generation through polarity control, microbe exposure, transplantation, and multi-organ boundary models.
Strong supporting sources
Working synthesis
- McCracken and Broda provide the developmental starting points for intestinal and gastric identity from pluripotent cells.
- Puschhof, Co, and Watanabe show how intestinal organoids become experimentally useful when lumen access, polarity, host interaction, or transplantation are built in.
- Koike and Broutier broaden endodermal work toward boundary models and adult self-renewing tissue systems.
Main tension
- developmental derivation versus downstream assay engineering
- simple epithelial models versus boundary-connected or repair-oriented systems
Open questions
- When is a baseline gut or gastric protocol enough, and when is polarity control or transplantation essential?
- How far can endodermal protocols be pushed toward multi-organ connectivity without sacrificing robustness?