Current position in this corpus
A major trend across this collection is the move away from simple epithelial balls toward organoids with multiple coordinated lineages, appendages, or anatomical boundary relationships.
Strong supporting sources
Working synthesis
- Lancaster, Lee, and some cardiac papers show the value of emergent tissue complexity.
- Koike and Dardano explicitly pursue linked developmental programs across neighboring organs or hematopoietic compartments.
- Olijnik and Matkovic Leko push organoid logic into niche or disease contexts where multicellular composition is central to the model.
Main tension
- biological richness versus protocol simplicity and scalability
- compositional realism versus clean mechanistic interpretation
Open questions
- Which extra tissue compartments materially change the answer to the biological question?
- When does added complexity improve realism, and when does it mostly increase variability?